© Benaki Phytopathological Institute
Nematicidal effect of entomopathogenic nematodes and garlic components
23
survive the first application hatch into in-
fective juveniles, which are subsequent-
ly killed by the second application. Alterna-
tively, due to the high volatility of the diallyl
disulfide and the protective nature of the
egg mass (11), repeated applications are re-
quired to achieve higher effectiveness. It is
also worth mentioning that neither the sin-
gle nor the double application of diallyl dis-
ulfide caused any phytotoxicity.
It can be concluded that both
S. car-
pocapsae
and diallyl disulfide exhibit signifi-
cant nematicidal or nematostatic properties
and have the potential for nematode con-
trol. However, more parameters should be
studied, such as plant parasitic nematode
initial infestation density, soil type, applica-
tion time, dosage and repeated applications
of diallyl disulfide.
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Table 1.
Effects of diallyl disulfide (Dd), used alone or in combination with dead or live
Stein-
ernema carpocapsae
(Sc), on the number of female
Meloidogyne javanica
(
Mj
), 28 days after
inoculation of potted tomato plants (cv. ‘Belladona’).
Treatment
Rate/dose
Time of application (dpi)
1
Nematodes per root
2
Control
0
0
243 a
3
Dd
2 μl/ml
0
112.8 cd
Dd
2 & 2 μl/ml
0 & 7
76.4 def
Live Sc
7,000 IJ
0
165.4 b
Dead Sc
7,000 IJ
0
133 bc
Live Sc + Dd
7,000 IJ + 2 μl/ml
0
104.2 cde
Live Sc + Dd
7,000 IJ + (2 & 2 μl/ml)
0 + (0 & 7)
71.2 ef
Dead Sc + Dd
7,000 IJ + 2 μl/ml
0
101 cde
Dead Sc + Dd
7,000 IJ + (2 & 2 μl/ml)
0 + (0 & 7)
59.4 f
LSD = 40
1
dpi: days post-
Mj
inoculation
2
Mean of five replicates
3
Numbers followed by the same letter do not differ significantly at P<0.001
1...,15,16,17,18,19,20,21,22,23,24 26,27,28,29,30,31,32,33,34