Page 53 - Volume 1, Issue 1, January 2008
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Phytophthora primulae
51
Table 1.
Susceptibility of different plant species to
Phytophthora primulae.
Plant species
Phytophthora primulae
BPIC2584
Disease index
Petroselinum crispum
3.93
Lycopersicon esculentum
hybrid Belladona
1
Lactuca sativa
cv. Paris Island Cos
1
Brassica oleracea
var.
botrytis
cv. Alpha
1
Brassica oleracea
var.
asparagoides
cv. Ramoso Calabrese
1
Brassica oleracea
var.
capitata
cv. Langedijk autumn
1
Brassica oleracea
var.
capitata
cv. Merveille d’ Octobre
1
Allium porrum
cv. Blue solaise
1
Brassica oleracea
var.
gemmifera
cv. Groninger
1
Daucus carota
cv. Nantes
1
Primula acaulis
1
Disease index (average of 15 replications), 1: no infection, 5: 100% plants dead or almost dead.
Fig. 5.
PCR amplified products from ribosomal DNA, using the ITS6 and ITS4 primers, were digested with the restric-
tion enzymes
Alu
I (A),
Msp
I (B) and
Taq
I (C) for RFLP (Restriction Fragment Length Polymorphism) patterns, obtained
by gel electrophoresis. The same digest patterns were generated by BPIC2584 isolate of
P. primulae
(2) and BPIC1989
of
P. porri
(3) while the BPIC2514 isolate of
P. syringae
(1) showed a different distinctive pattern, L: the size marker is
100 bp ladder.
A
C
B
L 1 2 3
1 2 3 L
L 1 2 3
